Early Insight Into In Vivo Recellularization of Cell-Free Allogenic Heart Valves

Sarikouch, Samir; Theodoridis, Kaolina; Hilfiker, Andres; Boethig, Dietmar; Laufer, Günther; Andreas, Martin; Cebotari, Serghei ORCID; Tudorache, Igor; Bobylev, Dmitry; Neubert, Lavinia; Teiken, Kristin; Robertus, Jan Lukas; Jonigk, Danny; Beerbaum, Philipp ORCID; Haverich, Axel; Horke, Alexander

BACKGROUND: Unlike the vast amount of animal data available on the recellularization of allogenic decellularized heart valves (DHVs), there have only been sporadic histologic reports on such grafts in humans. METHODS: Two experienced cardiac pathologists independently evaluated human specimens obtained during reoperation between December 2010 and April 2017 DHVs in seven categories after automated staining (scores: 0 to 6) in comparison with published data. An optimal result of 42 points was classified as 100%. RESULTS: We found that 364 DHVs, 236 decellularized pulmonary homografts (DPHs), and 128 decellularized aortic homografts (DAHs) were implanted, and freedom from explantation was 96.1% (DAH) and 98.7% (DPH). Reoperations were because of (suspected) endocarditis in 5 of 11 patients, stenosis at the subvalvular or valvular or supravalvular level in 3 of 11 patients, planned staged reoperation in 2 of 11 patients, and 1 heart transplantation. Good reader agreement was reflected by an interagreement weighted kappa of 0.783 (95% confidence interval: 0.707 to 0.859). The relative histologic score in nonendocarditis specimens was 76% +/- 4.3% (maximum 81%). Intracellular procollagen type 1 production was found in recipient mesenchymal cells within the transplanted grafts. In endocarditis specimens the histologic score was significantly lower with 48% +/- 7.3% (minimum 41%, p = 0.0004) because of leucocyte infiltration and matrix degradation. One DPH showed immune system-mediated graft failure. Grafts obtained during the first 12 months after implantation were not evenly repopulated with less recellularization in the inner parts; no difference was found between DAH and DPH with respect to extent of recellularization. CONCLUSIONS: Substantial in vivo recellularization with noninflammatory cells was observed in this study. Spontaneous recellularization appears to require multiple months, which correspondingly has an impact on size selection for growing patients

Cite

Citation style:

Sarikouch, Samir / Theodoridis, Kaolina / Hilfiker, Andres / et al: Early Insight Into In Vivo Recellularization of Cell-Free Allogenic Heart Valves. 2019.

Access Statistic

Total:
Downloads:
Abtractviews:
Last 12 Month:
Downloads:
Abtractviews:

open graphic

Rights

Use and reproduction:

Export