PIL012_Auswertung_Schmerzevaluierung_Piritramid_LZ_05-2024.xlsx

Data concerning the following project/manuscript:

Oral Self-Administration of Piritramide in C57BL/6JRj Mice Following Laparotomy: Cage-Side Pain Assessment Evaluation   

Lena Zachhuber, Claudia Kuntner, Thomas Wanek, Aylina Glasenapp, Marion Bankstahl

Despite its clinical use, there is a lack of comprehensive data on the analgesic potential of piritramide for post-surgical pain management in laboratory animals. Therefore, this study investigated the potential of a combined therapy of orally administered piritramide and subcutaneously injected buprenorphine for post-surgical pain in C57BL/6JRj mice after laparotomy. Male and female mice received a preoperative buprenorphine injection, followed by oral self-administration of piritramide in drinking water for four days post-surgery. Physiological and behavioral parameters i.e., water and food intake, nesting behavior, voluntary wheel running activity, body weight, and clinical score, were monitored. Results indicated no significant decrease in water intake in female mice, whereas male mice had a significant reduction 4-7 hours after surgery (p = 0.0229). Dry food intake remained below baseline consumption, but a higher intake of wet food compensated for this. Nesting behavior, wheel running time and distance showed no significant impairment. However, male mice exhibited increased running velocity at 104 hours post-surgery (p = 0.0113). Minimal weight loss and mild increased clinical scores were observed. These findings suggest that piritramide, in combination with preoperative buprenorphine, exhibits analgesic potential and was well-tolerated, supporting its applicability for management of laparotomy-induced pain  in laboratory mice.

Data were aquired at Medical University of Vienna, by Lena Zachhuber et al.